Ultrabright Labeling for Single-Neuron Reconstruction

We developed an ultra-bright chemical labeling method (LINCS) and AAV-based sparse expression systems for efficient brain-wide single neuron reconstruction.

[LINCS paper / Protocol / Addgene] [Dual-AAV Sparse Labeling Paper / Addgene]

By directed evolution, we developed a series of AAV vectors, AAV-cMG & AAV-MG, for microglial transgene delivery.

[Paper] [Addgene]

Microglia-effective AAVs

High-throughput Biomolecule Detection

We developed high-throughput immunoassays (isHCR, misHCR & BLISA) using modular antibody DNA-barcoding (MaMBA) for multiplexed in situ protein imaging and high-throughput biomolecule detection.

[MaMBA paper] [isHCR paper]